LOCUS       40924_23664       12156 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB702omega DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12156)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12156)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1083
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1091..1157
                     /label=omega translational enhancer
                     /note="omega translational enhancer"
     protein_bind    1172..1296
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1333..1363
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1417..2073
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2418..2720
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2764..2888)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2920..3172
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3206..3222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3230..3246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3254..3284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3299..3320)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3356..3608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3646..4941)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61164.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3646..4941)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4993..5172)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5678..5702)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6223..7011
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7260..7848
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8034..8174)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8518..8712)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8781..9851)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10283..10909)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
      661 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      721 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      781 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      841 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      901 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      961 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1021 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     1081 tgaaatcacc agtgtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     1141 caattactat ttacaatttc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1201 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1261 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1321 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1381 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1441 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1501 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1561 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1621 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1681 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1741 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1801 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1861 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1921 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1981 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2041 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     2101 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2161 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2221 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2281 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2341 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2401 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2461 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2521 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2581 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2641 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2701 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2761 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     2821 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     2881 aaagtggttg ataacagcgc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     2941 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3001 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3061 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3121 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3181 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3301 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     3361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     3481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     3541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     3601 gaacgatcgg ggaaattcgc ctcgagtgac ttctctgatt gcagactatt tataccaacc
     3661 agcaagaaag tgtcgaagta tgaagcagaa ggcgcaggct agagcctgga aaacgagaag
     3721 atggatgcaa agagactttt ctgatttagg accaaacaac ctcaagtaaa cattgacgag
     3781 tgtcccatct tttgtgccgg tcagtaatcc ggtagcagga tcataccttg ggagacgatg
     3841 gcgcggacat ttcacaatcc cagcaagctg aggaagcgat aatagaaggt tcagaacttg
     3901 aggaagaaag aagatgagga gggtttcact gaagtgaccc agaataccaa caactgccat
     3961 agtcattccg gcgaagactg tgtaagtgtc tccaacaaaa actgaagaag gataccaatt
     4021 gtaagcaagc attgccaagg atgtggccat cagcggttga gtaagaaata tcgagaaggc
     4081 atgagcttgg tgatactcgg gatcaacaga tgctccgatt tgcataacgt tgtgtatcag
     4141 gatagcagcg gcaataacaa ctgtttgacc gatttcaagg ccattcagac cagcgtggat
     4201 gttaatagag tttgtacaaa agacagcaag tagccccata tataacttat atatccttcc
     4261 tagattaagc acttccaagc caatataagc aacaagaggt tttggtataa caatagttgt
     4321 atgaccagca taagccatca aaagtggaag ggtagcaaaa gatggcagga caagttttac
     4381 tctccaaggc acatcaagga catcatctac aaatccaagc aaaatcatga agcaaataga
     4441 tgctagtgct gcattatact ccacaagcca attagaatct tcagtgaagt taaaatactg
     4501 aaatattatc gccacgatca aaaagacaat accaacaaca atacccaatg actctggcac
     4561 tttaatatct ccttgaggcg tgcctctttt gttgatatca aaaccaaaca tgttacgtct
     4621 cagaacgtat ctggcagtaa caggaatcat tttgagagtg acgaaaaacc caaccaagct
     4681 aagccccgcg ttaattagaa tcgatcgctt gagttcatta tcaaccttgt agtgaaaaca
     4741 gagaaggtat agatagagcg aacagagaag agtagagatc acgaagatca ctcctaattt
     4801 cggcggcgcg agacgaaatt cctcaccgga agcagaaacc gtctttcggg tcatcttctg
     4861 ctccgatggc gctgagttgg gttccgacgg gtccggtttg ttgggaattg agatcgatga
     4921 agctctctta cgagctgcca tatcttattg ccccccggga tcagatctgg attgagagtg
     4981 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     5041 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     5101 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
     5161 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     5221 cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct
     5281 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     5341 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     5401 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     5461 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
     5521 gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc
     5581 gtcgagatta atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg
     5641 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     5701 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     5761 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     5821 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     5881 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     5941 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     6001 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     6061 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     6121 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     6181 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     6241 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     6301 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     6361 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     6421 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     6481 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     6541 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     6601 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     6661 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     6721 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     6781 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     6841 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     6901 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     6961 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     7021 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     7081 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     7141 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     7201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7381 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7921 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7981 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     8041 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     8101 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     8161 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8221 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     8281 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     8341 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     8401 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     8461 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     8521 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     8581 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     8641 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     8701 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     8761 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     8821 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     8881 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     8941 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     9001 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     9061 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     9121 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     9181 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     9241 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     9301 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     9361 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     9421 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     9481 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     9541 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     9601 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     9661 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     9721 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     9781 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     9841 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     9901 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     9961 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
    10021 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
    10081 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
    10141 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
    10201 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
    10261 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
    10321 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
    10381 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
    10441 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
    10501 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
    10561 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
    10621 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
    10681 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
    10741 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
    10801 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
    10861 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
    10921 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
    10981 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
    11041 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
    11101 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
    11161 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
    11221 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
    11281 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
    11341 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
    11401 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
    11461 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
    11521 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
    11581 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
    11641 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
    11701 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
    11761 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
    11821 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
    11881 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
    11941 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
    12001 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
    12061 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
    12121 tggcatgcac atacaaatgg acgaacggat aaacct
//
